EFFECTS OF p16INK4 GENE ON CHEMOSENSITIVITY OF HUMAN GLIOMA U251 CELL LINE TO TENIPOSIDE
Abstract
Objective: To determine the effects on the cell growth, tumorigenicity and chemoseusitivity of pl6/CDK4I in human glioma.
Methods: p16 gene was transfected into U251 cells by lipofectin. Expression of exogenous p16 gene was confirmed by immunohistodaemistry and Northern blot. The effects of exogenous p16 gene on the growth and chemosensitivity to teniposide were examined.
Results: Expression of exogenous p16 gene inhibited the growth dramatically in vitro. G1 arrest of tumor cells was observed. However, wt pl6-positive U251 was less sensitive than control cell lines and the number of apoptotic cells after chemotherapy was reduced.
Conclusion: The expression of exogenous p16 gene could inhibit the growth of glioma. On the other hand, the chemosensitivity to teniposide of p16- positive U251 was decreased.
Methods: p16 gene was transfected into U251 cells by lipofectin. Expression of exogenous p16 gene was confirmed by immunohistodaemistry and Northern blot. The effects of exogenous p16 gene on the growth and chemosensitivity to teniposide were examined.
Results: Expression of exogenous p16 gene inhibited the growth dramatically in vitro. G1 arrest of tumor cells was observed. However, wt pl6-positive U251 was less sensitive than control cell lines and the number of apoptotic cells after chemotherapy was reduced.
Conclusion: The expression of exogenous p16 gene could inhibit the growth of glioma. On the other hand, the chemosensitivity to teniposide of p16- positive U251 was decreased.