LACKING EXON5 OF VARIANT ESTROGEN RECEPTOR IN HUMAN BREAST CANCER
Abstract
Methods: The target sequence of ER RNA covering exon4-6(1082-1520bp) was amplified in 7 clinical human breast cancer tissues by reverse transcription and polymerase chain reaction (RT-PCR) techniques.
Results: PCR products were transferred to nitrocellulose membranes and hybridized using a [r-32P]-ATP labeled ER 29 oligonulceotide probe representing the antisense strand of the ER cDNA sequence 1271-1299. Specific bands were found at 438 and 300 base pairs in two tumors. The 300 base pair of PCR product was recovered from ER+/PR+ and ER+/PR- tumor, respectively.
Conclusion: Dideoxy sequence analysis revealed that they contained the variant ER completely missing exon 5.
Results: PCR products were transferred to nitrocellulose membranes and hybridized using a [r-32P]-ATP labeled ER 29 oligonulceotide probe representing the antisense strand of the ER cDNA sequence 1271-1299. Specific bands were found at 438 and 300 base pairs in two tumors. The 300 base pair of PCR product was recovered from ER+/PR+ and ER+/PR- tumor, respectively.
Conclusion: Dideoxy sequence analysis revealed that they contained the variant ER completely missing exon 5.