GENERATION OF MONOCLONAL ANTIBODY AGAINST HUMAN ANDROGEN RECEPTOR WITH SYNTHETIC PEPTIDE
Abstract
Objective: Preparation of anti-human androgen receptor(hAR) monoclonal antibody (McAb).
Methods: Four cells lines of hybridoma secreting specific monocional antibodies against AR were first established by fusion SP2/0 cell with spleen cell from BALB/c mice immunized with the coupling complex of hAR-KLH.
Results: Paraffin-embedded sections of 45 prostate cancers were detected. There was an overall concordance of 91% using Immunohistochemistry between AR polyclonal antibody from Zymed and hAR-N McAb selfmade.
Conclusion: The results show that the McAb obtained in this study would be a useful tool to detect the AR status in prostate cancer.
Methods: Four cells lines of hybridoma secreting specific monocional antibodies against AR were first established by fusion SP2/0 cell with spleen cell from BALB/c mice immunized with the coupling complex of hAR-KLH.
Results: Paraffin-embedded sections of 45 prostate cancers were detected. There was an overall concordance of 91% using Immunohistochemistry between AR polyclonal antibody from Zymed and hAR-N McAb selfmade.
Conclusion: The results show that the McAb obtained in this study would be a useful tool to detect the AR status in prostate cancer.