Article Abstract

INHIBITION OF TELOMERASE ACTIVITY DURING INDUCTION OF HL-60 CELLS BY RETINOID Ro13-7410

Authors: Xiao-shan Liu,Ling-sheng Lou,Ji-kai Jiang,Bei-zhong Liu,Jian-fang Zhou,Zong-shan Tang,Xue-xian Li,Ge-fei Kang

Abstract

Objective: To investigate the effects of R013-7410 on telomerase activity and cell cycle distribution.

Methods: Telomerase activity of HL-60 cells induced by retinoid Ro13-7410 was detected by telomerase PCRELISA- kit. The cell cycle was analyzed by flow cytometry.

Results: Telomerase activity declined gradually after 10 .6 mol/L Ro13-7410 treatment, and the inhibition of telomerase activity at day 5 of treatment with Ro13- 7410 was less effective than with Retinoid Acid (RA). DNA flow cytofluorimetric analysis revealed that Ro13- 7410 caused partial cells arrest in the G2/M phase after 4-days treatment.

Conclusion: Telomerase activity declined gradually and partial cells were arrested in the G2/M phase after Ro13-7410 treatment.

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