Article Abstract

CLONING AND DETERMINING OF BAC GENE AND Bcl-2 AND CDK4 EXPRESSION ON ASCITES HEPATOMA CELL LINE Hca-F25/25CL-16A3

Authors: Yun-fei Zuo,Yao-zheng Zhang

Abstract

Objective: To study the mechanism of cancer, the DNA for BAC was cloned from an ascites hepatoma cell line Hca-F25/CL-16A3 using PCR.

Methods: The nucleotide sequences were determined using ABI PRISM TM 377 DNA sequencer. The expression of bcl-2 and CDK4 gene were determined using immunohistochemistry.

Results: The sequences of BAC segment on Hca- F25/CL-16A3 have nearly identical sequences with human BAC. The bcl-2 and CDK4 are highly expression on this cell line.

Conclusion: The highly expression of bcl-2 and CDK4 may the one of mechanisms for tumor growth.

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