Expressions of Poly (ADP-ribose) Glycohydrolase (PARG) and Membrane Type 1 Matrix Metalloproteinase (MT1-MMP) in Colorectal Carcinoma
Abstract
Objective: To investigate the significance of Poly (ADP-ribose) glycolhydrolase (PARG) and membrane type 1 matrix metalloproteinase (MT1-MMP) expressions in human colorectal carcinoma.
Methods: Immunohistochemical staining for PARG and MT1-MMP was carried out on colorectal adenoma-carcinoma tissue microarrays containing normal colorectal mucosae, adenoma, adenoma with malignant transformation and adenocarcinoma (total 130 specimens). The expressions of PARG and MT1-MMP in the GLTN [Gallotannin]-treated and GLTN-untreated lovo cells were detected by Western Blot.
Results: PARG expression in adenocarcinoma (83.1%) and adenoma with malignant transformation (66.7%) was significantly higher than that in normal colorectal mucosa (10%) and adenoma (10.5%). Expression of MT1-MMp in normal colorectal mucosa and adenoma was negative, while the expression in adenocarcinoma (80.3%) and adenoma with malignant transformation (72.2%) was high. The expressions of PARG and MT1-MMP in adenocarcinoma with metastasis and in late tumor stages were significantly higher than those in adenocarcinoma with no metastasis and in early tumor stages. Thus, PARG expression shows a positive correlation with the expression of MT1-MMP. The expressions of PARG and MT1-MMP in GLTN-treated lovo cells were weaker than that in GLTN-untreated lovo cells.
Conclusion: The expression of PARG was probably related to the development of colorectal carcinoma. PARG may play an important role for the regulation of MT1-MMP expression in colorectal carcinoma.
Methods: Immunohistochemical staining for PARG and MT1-MMP was carried out on colorectal adenoma-carcinoma tissue microarrays containing normal colorectal mucosae, adenoma, adenoma with malignant transformation and adenocarcinoma (total 130 specimens). The expressions of PARG and MT1-MMP in the GLTN [Gallotannin]-treated and GLTN-untreated lovo cells were detected by Western Blot.
Results: PARG expression in adenocarcinoma (83.1%) and adenoma with malignant transformation (66.7%) was significantly higher than that in normal colorectal mucosa (10%) and adenoma (10.5%). Expression of MT1-MMp in normal colorectal mucosa and adenoma was negative, while the expression in adenocarcinoma (80.3%) and adenoma with malignant transformation (72.2%) was high. The expressions of PARG and MT1-MMP in adenocarcinoma with metastasis and in late tumor stages were significantly higher than those in adenocarcinoma with no metastasis and in early tumor stages. Thus, PARG expression shows a positive correlation with the expression of MT1-MMP. The expressions of PARG and MT1-MMP in GLTN-treated lovo cells were weaker than that in GLTN-untreated lovo cells.
Conclusion: The expression of PARG was probably related to the development of colorectal carcinoma. PARG may play an important role for the regulation of MT1-MMP expression in colorectal carcinoma.